全文阅读
本文目的是用免疫组织化学方法显示催产素在卵泡壁细胞中和幼稚黄体中的定位.实验用3头性周期正常的母牛.分别于性周期的第6天和7～17天之间以及性周期末摘取卵巢.于显微镜下确定黄体.将组织块分别固定于Bouin氏液及戊二醛苦味酸及冰醋酸混合液(GPA)中6或24小时.固定后常规石蜡包埋,切片厚5μm用于免疫细胞化学染色.相邻切片HE染色.切片按Sternberger(1979)间接过氧化物酶-抗过氧化物酶(PAP)法进行免疫细胞化学染色.第一抗体用兔抗合成的催产素抗血清,稀释度为1:500.第二抗体用驴抗兔抗血清.
The purpose of this paper is to show the location of oxytocin in follicular parietal cells and naive luteal cells by immunohistochemical methods.Experimental use of 3 cows with normal sexual cycles were carried out on days 6 and 7 to 17 of the sexual cycle and Ovaries were taken at the end of the sexual cycle and luteal cells were identified under a microscope.The tissue pieces were fixed in Bouin’s solution and glutaraldehyde picric acid and glacial acetic acid mixture (GPA) for 6 or 24 hours, respectively, fixed and embedded in paraffin, sliced Thickness 5μm for immunocytochemistry staining of adjacent sections HE staining sections according to Sternberger (1979) indirect peroxidase - anti-peroxidase (PAP) method of immunocytochemical staining of the first antibody rabbit anti-synthetic Oxytocin antiserum, dilution 1: 500. The second antibody donkey anti-rabbit antiserum.